| First Author | Muraleedharan CK | Year | 2019 |
| Journal | J Innate Immun | Volume | 11 |
| Issue | 4 | Pages | 347-358 |
| PubMed ID | 30625496 | Mgi Jnum | J:285683 |
| Mgi Id | MGI:6399996 | Doi | 10.1159/000495472 |
| Citation | Muraleedharan CK, et al. (2019) The miR-183/96/182 Cluster Regulates Macrophage Functions in Response to Pseudomonas aeruginosa. J Innate Immun 11(4):347-358 |
| abstractText | Macrophages (Mvarphi) are an important component of the innate immune system; they play critical roles in the first line of defense to pathogen invasion and modulate adaptive immunity. MicroRNAs (miRNAs) are a newly recognized, important level of gene expression regulation. However, their roles in the regulation of Mvarphi and the immune system are still not fully understood. In this report, we provide evidence that the conserved miR-183/96/182 cluster (miR-183/96/182) modulates Mvarphi function in their production of reactive nitrogen (RNS) and oxygen species (ROS) and their inflammatory response to Pseudomonas aeruginosa (PA) infection and/or lipopolysaccharide (LPS) treatment. We show that knockdown of miR-183/96/182 results in decreased production of multiple proinflammatory cytokines in response to PA or LPS treatment in Mvarphi-like Raw264.7 cells. Consistently, peritoneal Mvarphi from miR-183/96/182-knockout versus wild-type mice are less responsive to PA or LPS, although their basal levels of proinflammatory cytokines are increased. In addition, overexpression of miR-183/96/182 results in decreased production of nitrite and ROS in Raw264.7 cells. We also provide evidence that DAP12 and Nox2 are downstream target genes of miR-183/96/182. These data suggest that miR-183/96/182 imposes global regulation on various aspects of Mvarphi function through different downstream target genes. |
