| First Author | Sun L | Year | 2021 |
| Journal | Prog Neurobiol | Volume | 202 |
| Pages | 102024 | PubMed ID | 33636225 |
| Mgi Jnum | J:358208 | Mgi Id | MGI:7778471 |
| Doi | 10.1016/j.pneurobio.2021.102024 | Citation | Sun L, et al. (2021) MicroRNA-96 is required to prevent allodynia by repressing voltage-gated sodium channels in spinal cord. Prog Neurobiol 202:102024 |
| abstractText | Voltage-gated sodium channels (Na(v)s) 1.7, 1.8, and 1.9 are predominately expressed in peripheral sensory neurons and are critical for action potential propagation in nociceptors. Unexpectedly, we found that expression of SCN9A, SCN10A, SCN11A, and SCN2A, the alpha subunit of Na(v)1.7, Na(v)1.8, Na(v)1.9 and Na(v)1.2, respectively, are up-regulated in spinal dorsal horn (SDH) neurons of miR-96 knockout mice. These mice also have de-repression of CACNA2D1/2 in DRG and display thermal and mechanical allodynia that could be attenuated by intrathecal or intraperitoneal injection of Na(v)1.7 or Na(v)1.8 blockers or Gabapentin. Moreover, Gad2::CreERT2 conditional miR-96 knockout mice phenocopied global knockout mice, implicating inhibitory neurons; nerve injury induced significant loss of miR-96 in SDH GABAergic and Glutamatergic neurons in mice which negatively correlated to up-regulation of Na(v)1.7, Na(v)1.8, Na(v)1.9 and Scn2a, this dis-regulation of miR-96 and Na(v)s in SDH neurons contributed to neuropathic pain which can be alleviated by intrathecal injection of Na(v)1.7 or Na(v)1.8 blockers. In conclusion, miR-96 is required to avoid allodynia through limiting the expression of VGCCs and Na(v)s in DRG and Na(v)s in SDH in naive and nerve injury-induced neuropathic pain mice. Our findings suggest that central nervous system penetrating Na(v)1.7 and Na(v)1.8 blockers may be efficacious for pain relief. |
