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Allele : Tg(CAG-Bgeo,-SMN2)E9Dscd transgene insertion E9, Dawn S Chandler

Primary Identifier  MGI:5427354 Allele Type  Transgenic
Attribute String  Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter Gene  Tg(CAG-Bgeo,-SMN2)E9Dscd
Transmission  Germline Strain of Origin  129S6/SvEvTac
Is Recombinase  false Is Wild Type  false
molecularNote  The pCCALL2/hSMN2 transgene was designed with, from 5' to 3', the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (originally from the pCAGG vector), a loxP-flanked STOP cassette (beta-galactosidase/neomycin resistance cassette [beta-geo] with three copies of the SV40 polyA signal), the 1.37 kbp full-length human SMN2 cDNA sequence including all exons (1-8) from the start codon to the stop codon, and a rabbit polyA signal. The 1.37 kbp human SMN2 cDNA sequence (encoding exons 1-8) was originally derived via RT-PCR performed on total RNA isolated from SMA type 1-derived patient fibroblast cells (Coriell Institute GM 3813; shown to express both full-length and truncated SMN2 transcripts). Lines A9 and E9 was generated.
  • mutations:
  • Insertion
  • synonyms:
  • Tg hSMN2 E9,
  • Tg hSMN2 E9
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

0 Driven By

3 Publication categories

Trail: Allele