| Primary Identifier | MGI:1857866 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Hnf1b |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| description | Complementation of Tcf2tm1Mya mutant embryos with wild-type visceral endoderm by tetraploid aggregation extends viability to E10 and rescues gastrulation and the growth defect up to E9.5; however, at E9.5-E10.0, tetraploid-rescued embryos are shorter and resemble E8.5 embryos. Additional phenotypes include: incomplete embryo turning, failure to close the neural tube, neural tissue overgrowth associated with edema on both neural folds and on forebrain vesicles, ruffling of the visceral endoderm, an enlarged allantois, distended pericardia, and failure to close and/or invaginate the gut (J:58080). Injection of mutant ES cells into wild-type blastocysts results in chimeric animals with a closed neural tube and a normal mesonephros; however, mutant ES cells fail to contribute to gut formation, as shown by absence of X-gal staining (J:58080). |
| molecularNote | Exon 1 was replaced by the insertion of NLS-lacZ and neo. Western blot analysis of total RNA isolated from homozygous differentiated ES cells revealed an absence of transcript. Expression of beta-galactosidase was controlled by the endogenous promoter. |
