Primary Identifier | MGI:7286051 | Allele Type | Endonuclease-mediated |
Attribute String | Epitope tag, Recombinase | Gene | Hpse |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | true | Is Wild Type | false |
molecularNote | Using CRISPR/Cas9 technology, a P2A-Cre (encoding a viral 2A oligopeptide that mediates ribosomal skipping and a codon-optimized cre gene) and a myc-tag were inserted into the targetted STOP codon of the Heparanase gene (Hpse) on chromosome 9 in embryonic stem cells. Chimeric mice were generated by aggregating ES cells with morula-stage embryos and bred for germline transmission. |