| First Author | Rougeon F | Year | 1977 |
| Journal | Gene | Volume | 1 |
| Issue | 3-4 | Pages | 229-39 |
| PubMed ID | 338418 | Mgi Jnum | J:5902 |
| Mgi Id | MGI:54379 | Doi | 10.1016/0378-1119(77)90047-6 |
| Citation | Rougeon F, et al. (1977) Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro. Gene 1(3-4):229-39 |
| abstractText | New chimeric Escherichia coli plasmids containing alpha or beta globin gene sequences of the mouse were constructed. Double-stranded DNA, synthesised in vitro in a 2-step reaction from mouse globin mRNA was inserted into E. coli plasmid pCR1, after tailing of the 2 DNAs with dG and dC respectively. Some of the mouse globin plasmids described contain at least 90% of the globin mRNA sequence and therefore contain the entire translated sequence of the globin genes. Some possible uses of these recombinant plasmids are described. |
