| First Author | Mujalli A | Year | 2023 |
| Journal | Biomolecules | Volume | 13 |
| Issue | 4 | PubMed ID | 37189331 |
| Mgi Jnum | J:335856 | Mgi Id | MGI:7482917 |
| Doi | 10.3390/biom13040583 | Citation | Mujalli A, et al. (2023) Exploring the Role of PI3P in Platelets: Insights from a Novel External PI3P Pool. Biomolecules 13(4) |
| abstractText | Phosphoinositides (PIs) play a crucial role in regulating intracellular signaling, actin cytoskeleton rearrangements, and membrane trafficking by binding to specific domains of effector proteins. They are primarily found in the membrane leaflets facing the cytosol. Our study demonstrates the presence of a pool of phosphatidylinositol 3-monophosphate (PI3P) in the outer leaflet of the plasma membrane of resting human and mouse platelets. This pool of PI3P is accessible to exogenous recombinant myotubularin 3-phosphatase and ABH phospholipase. Mouse platelets with loss of function of class III PI 3-kinase and class II PI 3-kinase alpha have a decreased level of external PI3P, suggesting a contribution of these kinases to this pool of PI3P. After injection in mouse, or incubation ex vivo in human blood, PI3P-binding proteins decorated the platelet surface as well as alpha-granules. Upon activation, these platelets were able to secrete the PI3P-binding proteins. These data sheds light on a previously unknown external pool of PI3P in the platelet plasma membrane that recognizes PI3P-binding proteins, leading to their uptake towards alpha-granules. This study raises questions about the potential function of this external PI3P in the communication of platelets with the extracellular environment, and its possible role in eliminating proteins from the plasma. |
