| First Author | Almandoz-Gil L | Year | 2018 |
| Journal | Front Neurol | Volume | 9 |
| Pages | 180 | PubMed ID | 29623065 |
| Mgi Jnum | J:337639 | Mgi Id | MGI:6756209 |
| Doi | 10.3389/fneur.2018.00180 | Citation | Almandoz-Gil L, et al. (2018) In Situ Proximity Ligation Assay Reveals Co-Localization of Alpha-Synuclein and SNARE Proteins in Murine Primary Neurons. Front Neurol 9:180 |
| abstractText | The aggregation of alpha-synuclein (alphaSyn) is the pathological hallmark of Parkinson's disease, dementia with Lewy bodies and related neurological disorders. However, the physiological function of the protein and how this function relates to its pathological effects remain poorly understood. One of the proposed roles of alphaSyn is to promote the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex assembly by binding to VAMP-2. The objective of this study was to visualize the co-localization between alphaSyn and the SNARE proteins (VAMP-2, SNAP-25, and syntaxin-1) for the first time using in situ proximity ligation assay (PLA). Cortical primary neurons were cultured from either non-transgenic or transgenic mice expressing human alphaSyn with the A30P mutation under the Thy-1 promoter. With an antibody recognizing both mouse and human alphaSyn, a PLA signal indicating close proximity between alphaSyn and the three SNARE proteins was observed both in the soma and throughout the processes. No differences in the extent of PLA signals were seen between non-transgenic and transgenic neurons. With an antibody specific against human alphaSyn, the PLA signal was mostly located to the soma and was only present in a few cells. Taken together, in situ PLA is a method that can be used to investigate the co-localization of alphaSyn and the SNARE proteins in primary neuronal cultures. |
