First Author | Porro F | Year | 2004 |
Journal | FEBS Lett | Volume | 576 |
Issue | 1-2 | Pages | 36-40 |
PubMed ID | 15474006 | Mgi Jnum | J:93294 |
Mgi Id | MGI:3056826 | Doi | 10.1016/j.febslet.2004.08.057 |
Citation | Porro F, et al. (2004) The erythrocyte skeletons of beta-adducin deficient mice have altered levels of tropomyosin, tropomodulin and EcapZ. FEBS Lett 576(1-2):36-40 |
abstractText | The erythrocyte membrane cytoskeleton is organized as a polygonal spectrin network linked to short actin filaments that are capped by adducin at the barbed ends. We have constructed a mouse strain deficient in beta-adducin having abnormal erythrocytes. We show here that the levels of several skeletal proteins from beta-adducin mutant erythrocytes are altered. In fact, CapZ, the main muscle actin-capping protein of the barbed ends that in the erythrocytes is cytoplasmic, is 9-fold upregulated in mutant skeletons of erythrocytes suggesting a compensatory mechanism. We also detected upregulation of tropomodulin and downregulation of alpha-tropomyosin and actin. In addition, purified adducin can be re-incorporated into adducin-deficient ghosts. |