| First Author | Ramirez JM | Year | 2004 |
| Journal | Virology | Volume | 330 |
| Issue | 2 | Pages | 398-407 |
| PubMed ID | 15567434 | Mgi Jnum | J:95484 |
| Mgi Id | MGI:3526203 | Doi | 10.1016/j.virol.2004.09.038 |
| Citation | Ramirez JM, et al. (2004) Activation of c-myb by 5' retrovirus promoter insertion in myeloid neoplasms is dependent upon an intact alternative splice donor site (SD') in gag. Virology 330(2):398-407 |
| abstractText | Alternative splicing in Mo-MuLV recruits a splice donor site, SD', within the gag that is required for optimal replication in vitro. Remarkably, this SD' site was also found to be utilized for production of oncogenic gag-myb fusion RNA in 100% of murine-induced myeloid leukemia (MML) in pristane-treated BALB/c mice. Therefore, we investigated the influence of silent mutations of SD' in this model. Although there was no decrease in the overall incidence of disease, there was a decrease in the incidence of myeloid leukemia with a concomitant increase in lymphoid leukemia. Importantly, there was a complete lack of myeloid tumors associated with 5' insertional mutagenic activation of c-myb, suggesting the specific requirement of the SD' site in this mechanism. |
