| Primary Identifier | MGI:7507528 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready, Humanized sequence, Inserted expressed sequence, Null/knockout | Gene | Ace2 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The coding sequence of the first translated exon of the mouse gene (exon 2) was replaced by an expression cassette containing a loxP site, a modified human ACE2 cDNA, loxP site, woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), SV40 polyA signal, and FRT-flanked PGK-neomycin resistance gene via CRISPR/Cas9 technology. The human ACE2 cDNA was modified to use the mouse Ace2 signal peptide and codon-optimized for a mouseThe first loxP site interrupts the 5â untranslated sequence of the mouse Ace2. |
