| First Author | Barton GM | Year | 2002 |
| Journal | Proc Natl Acad Sci U S A | Volume | 99 |
| Issue | 10 | Pages | 6937-42 |
| PubMed ID | 12011451 | Mgi Jnum | J:135481 |
| Mgi Id | MGI:3793918 | Doi | 10.1073/pnas.102645699 |
| Citation | Barton GM, et al. (2002) Positive selection of self-MHC-reactive T cells by individual peptide-MHC class II complexes. Proc Natl Acad Sci U S A 99(10):6937-42 |
| abstractText | If T cells require specific interactions with MHC-bound peptides during positive selection, then the specificities of T cells selected by one peptide should be distinct from those selected by another. We have examined positive selection of CD4 T cells in four strains of mice, each overexpressing a different peptide-1-A(b)(A(b)) complex. We show that a subset of CD4 T cells is selected by the overexpressed peptide and that the specificities of the CD4 T cells, as measured by reactivity to wild-type antigen-presenting cells, vary greatly depending on which peptide is overexpressed. These differences in specificity are mediated through positive selection not negative selection. Each of the four peptide-A(b) complexes appears to adopt a different conformation, and these differences correlate with the differences in reactivity. Our results suggest that individual peptide-MHC complexes positively select different subsets of self-MHC-reactive T cells and that the conformation of the peptide-MHC complex may contribute to this process. |
