| Primary Identifier | MGI:7646874 | Allele Type | Endonuclease-mediated |
| Gene | Dnmt3a | Strain of Origin | (C57BL/6N x C3H/HeN)F1 x C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Aspartic acid codons 525 (GAC) and 527 (GAT) in exon 14 were changed to alanine (GCC, GCT) (p.D525A, p.D527A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutations, in the ADD domain of the encoded peptide, reduce the affinity of the peptide to bind to H3K4me0. |
