| First Author | Honda K | Year | 2007 |
| Journal | Carcinogenesis | Volume | 28 |
| Issue | 10 | Pages | 2074-81 |
| PubMed ID | 17494055 | Mgi Jnum | J:125790 |
| Mgi Id | MGI:3759929 | Doi | 10.1093/carcin/bgm112 |
| Citation | Honda K, et al. (2007) Epidermal hyperplasia and papillomatosis in mice with a keratinocyte-restricted deletion of csk. Carcinogenesis 28(10):2074-81 |
| abstractText | The Src family kinases (SFKs) are believed to play critical roles in malignant transformation, as well as in growth, invasion and dissemination of neoplastic tissue. Inhibition of SFK-mediated signal transduction and activation of downstream targets inhibits tumor progression. To determine whether constitutive activity of SFK per se is sufficient to induce tumorigenesis in vivo, we have generated a mouse model with a keratinocyte-restricted deletion of the SFK-negative regulator csk (Csk-K5 mice). Even though expression levels of SFKs were lower in C-terminal Src kinase (Csk)-null keratinocytes, activity levels were higher than in control keratinocytes. At the age of 3 months, all Csk-K5 mice displayed signs of chronic inflammation in dermis and epidermal hyperplasia. About 19% of Csk-K5 mice (7 out of 36) developed papillomatous lesions. However, these lesions did not show any signs of neoplastic transformation over the next 8 months. Epidermal hyperplasia and hyperkeratosis in Csk-K5 mice were associated with an increased number of stem cells in the interfollicular epidermis, an increased proliferation of basal keratinocytes and a delayed terminal differentiation of the suprabasal keratinocytes. Our results clearly demonstrate that even though SFK-mediated signaling promotes tumor progression, elevated activity of SFKs in vivo alone is not sufficient to induce neoplastic transformation. |
