Primary Identifier | MGI:6153198 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Dagla |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 1 and a FRT-flanked neomycin resistance gene and a second loxP site were inserted downstream of exon 1. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination excised exon 1. Real-time PCR confirmed complete loss of mRNA transcript and immunohistochemistry confirmed the absence of protein in brain. |