First Author | Hennekes H | Year | 1990 |
Journal | Mol Gen Genet | Volume | 221 |
Issue | 1 | Pages | 33-6 |
PubMed ID | 2325630 | Mgi Jnum | J:10425 |
Mgi Id | MGI:58876 | Doi | 10.1007/BF00280364 |
Citation | Hennekes H, et al. (1990) Coding sequence and flanking regions of the mouse vimentin gene. Mol Gen Genet 221(1):33-6 |
abstractText | Using a polyclonal antibody, a cDNA clone coding for part of mouse vimentin was identified in a lambda gt11 expression library. DNA from this clone was used to screen a genomic library from Ehrlich Ascites Tumor cells for the mouse vimentin gene. A clone was found which contained the whole coding sequence and a large part of the 5'- and 3'-untranslated sequences. It was used to prepare a construct equivalent to a full-length cDNA clone. Extensive homologies to the vimentin sequence from other species were found for the coding and 3'-untranslated sequences and the promoter region. |