| First Author | Goto A | Year | 2001 |
| Journal | Biochem J | Volume | 354 |
| Issue | Pt 2 | Pages | 243-8 |
| PubMed ID | 11171100 | Mgi Jnum | J:67897 |
| Mgi Id | MGI:1931670 | Doi | 10.1042/0264-6021:3540243 |
| Citation | Goto A, et al. (2001) Drosophila mitochondrial transcription factor A (d-TFAM) is dispensable for the transcription of mitochondrial DNA in Kc167 cells. Biochem J 354(Pt 2):243-8 |
| abstractText | We have cloned cDNA encoding Drosophila mitochondrial (mt) transcription factor A (d-TFAM). RNA interference (RNAi) of d-TFAM by lipofection of haemocyte-derived Kc167 cells with double-stranded RNA reduced d-TFAM to less than 5% of the normal level. Reflecting the ability of TFAM to stabilize mtDNA, RNAi of d-TFAM reduced mtDNA to 40%. Nonetheless, transcription of the ND2 and ND5 genes and their mRNAs remained unchanged for 8 days of the duration of RNAi. We thus show that d-TFAM is not essential for the transcription of Drosophila mtDNA. |
