| Primary Identifier | MGI:3663358 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(CAG-DsRed*MST)1Nagy |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | Erratum publ Genesis 42:218 (2005) correction to original (2004) publication: The red fluorescent protein used is DsRed.MST, not DsRed.T3 as J:102837. DsRed.MST differs from DsRed.T3 in two amino acids. It has Alanine at positions 145 and 217. |
| molecularNote | This strain is a derivative of Tg(ACTB-Bgeo,-DsRed*MST). A transgenic construct containing a Red Fluorescent Protein gene (DsRed.MST) under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene and polyadenylation sites, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. Then the expression of lacZ was replaced with red fluorescent protein following in vitro Cre recombination. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. |
