Primary Identifier | MGI:6164306 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Dcaf17 |
Transmission | Germline | Strain of Origin | (C57BL/6NTac x 129S6/SvEvTac)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A FRT/loxP flanked neomycin cassette was inserted upstream of exon 4 in intron 3-4 in an opposite direction in relation to the target gene. A single loxP site was inserted downstream of exon 4. Cre-mediated recombination removed both the selection cassette and exon 4. cDNA sequencing revealed that excision of exon 4 leads to a frameshift mutation with concomitant premature stop codon after 59 residues. |