| Primary Identifier | MGI:3620978 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Blm |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cassette containing a single-chain reverse tetracycline-controlled trans-silencer (sc rtTS), a tetracycline-controlled transactivator (tTA), and a tetracycline responsive element (TRE) were introduced upstream of the translation start site in exon 2. The sc rtTS consists of two silencer moieties connected via a 36 amino acid linker. In the presence of doxycycline, the sc rtTS binds to tetO to suppress leaky expression of the locus. |
