| First Author | Mathisen PM | Year | 1999 |
| Journal | J Biol Chem | Volume | 274 |
| Issue | 44 | Pages | 31571-6 |
| PubMed ID | 10531361 | Mgi Jnum | J:58153 |
| Mgi Id | MGI:1346884 | Doi | 10.1074/jbc.274.44.31571 |
| Citation | Mathisen PM, et al. (1999) Visinin-like protein (VILIP) is a neuron-specific calcium-dependent double-stranded RNA-binding protein. J Biol Chem 274(44):31571-6 |
| abstractText | Double-stranded RNA-binding proteins function in regulating the stability, translation, and localization of specific mRNAs. In this study, we have demonstrated that the neuron-specific, calcium-binding protein, visinin-like protein (VILIP) contains one double-stranded RNA-binding domain, a protein motif conserved among many double-stranded RNA-binding proteins. We showed that VILIP can specifically bind double-stranded RNA, and this interaction specifically requires the presence of calcium. Mobility shift studies indicated that VILIP binds double-stranded RNA as a single protein-RNA complex with an apparent equilibrium dissociation constant of 9.0 x 10(-6) M. To our knowledge, VILIP is the first double-stranded RNA-binding protein shown to be calcium-dependent. Furthermore, VILIP specifically binds the 3'-untranslated region of the neurotrophin receptor, trkB, an mRNA localized to hippocampal dendrites in an activity-dependent manner. Given that VILIP is also expressed in the hippocampus, these data suggest that VILIP may employ a novel, calcium-dependent mechanism to regulate its binding to important localized mRNAs in the central nervous system. |
