| First Author | Zucchi I | Year | 1998 |
| Journal | Proc Natl Acad Sci U S A | Volume | 95 |
| Issue | 3 | Pages | 1079-84 |
| PubMed ID | 9448288 | Mgi Jnum | J:45654 |
| Mgi Id | MGI:1195809 | Doi | 10.1073/pnas.95.3.1079 |
| Citation | Zucchi I, et al. (1998) The rat gene homologous to the human gene 9-27 is involved in the development of the mammary gland. Proc Natl Acad Sci U S A 95(3):1079-84 |
| abstractText | We have developed a model system for studying differentiation in the mammary gland, by using two clonal cultures deriving from a rat breast adenocarcinoma. They differ in the ability to form domes, structures the significance of which is unknown. By using the subtractive cDNA library approach, we isolated a cDNA that is highly expressed in the dome-forming cells, and identical to the rat8 gene and highly homologous to the human 9-27 gene. Antisense treatment of the dome-forming cells specifically and reproducibly abolishes dome formation, while forced expression of the gene in non-dome-forming cells causes morphological changes suggestive of flat domes. In situ hybridization on rat tissues shows that the gene is expressed in epithelia, especially in those forming tubular structures, suggesting a relatedness between these structures and domes. Cytokeratin 8 and E cadherin are strongly expressed in the domes but not outside them, suggesting that the rat8 gene triggers the cells to express molecules that tighten the lateral connections between the cells; the process is likely to parallel that occurring during the differentiation of the mammary gland. |
