| First Author | Sivasankar B | Year | 2007 |
| Journal | Mol Immunol | Volume | 44 |
| Issue | 11 | Pages | 2978-87 |
| PubMed ID | 17296227 | Mgi Jnum | J:119703 |
| Mgi Id | MGI:3703169 | Doi | 10.1016/j.molimm.2006.12.025 |
| Citation | Sivasankar B, et al. (2007) CD59a deficient mice display reduced B cell activity and antibody production in response to T-dependent antigens. Mol Immunol 44(11):2978-87 |
| abstractText | CD59a is the primary regulator of membrane attack complex in mice. Recently, we have shown that CD59a-deficient (Cd59a(-/-)) mice exhibit enhanced CD4(+) T cell responses. Here, we explored the effects of CD59a on B cell function and antibody production. Contrary to our expectations, Cd59a(-/-) mice showed a decreased humoral immune response to a T cell dependent antigen, sheep red blood cells. We found that the decreased humoral immune response was associated with a reduction in plasma cell number in vivo and reduced ability to respond to stimuli during in vitro culture experiments. Using MLR studies in which purified wild type or Cd59a(-/-) CD4(+) T cells were mixed with purified B cells from each source, we found that the reduced B cell activation was largely due to the absence of CD59a on CD4(+) T cells. Furthermore, a CD59a fusion protein bound specifically to mouse B cells, and enhanced B cell proliferation in a MLR, demonstrating that B cells express an as yet unidentified ligand for CD59a that aids in B cell activation. |
