| Primary Identifier | MGI:3577336 | Allele Type | Targeted |
| Attribute String | Modified regulatory region, Null/knockout | Gene | Tcra-J |
| Transmission | Germline | Strain of Origin | 129S/SvEv x C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Tcra-Jtm2.1Krg ES cells (where the Trja49 promoter (Rr319) and gene segment were replaced with a loxP site flanked neomycin resistance gene cassette and the neo cassette was removed through subsequent cre-mediated recombination) were targeted with a vector replacing the TEA (T early alpha) promoter with a floxed neo cassette. The region between the loxP site in place of Trja49 and the upstream loxP site in place of the TEA promoter was deleted through subsequent cre-mediated recombination. This deletes segments Traj50-61 and the non-coding TEA exon. No gross deficiency was found in alphabeta TCR expression or alphabeta T cell development in mutant mice. |
