| First Author | Ozawa K | Year | 1990 |
| Journal | Nucleic Acids Res | Volume | 18 |
| Issue | 14 | Pages | 4185-90 |
| PubMed ID | 2377459 | Mgi Jnum | J:10653 |
| Mgi Id | MGI:59100 | Doi | 10.1093/nar/18.14.4185 |
| Citation | Ozawa K, et al. (1990) Comparison of the promoter regions of H-2Kb and H-2Kbm1 class I MHC genes. Nucleic Acids Res 18(14):4185-90 |
| abstractText | The 2.0 kb-long nucleotide sequences of the promoter regions of two closely related class I genes of the mouse major histocompatibility complex (H-2Kb and H-2Kbm1) have been determined and compared. The promoter sequence of the H-2Kbm1 gene differs from that of the H-2Kb gene by a single deletion of a 'C' at position -456 in the upstream region of H-2Kbm1 gene. The actual existence of this deletion of a single base in genomic DNA has been verified by genomic DNA hybridization, using oligonucleotide probes specific for H-2Kbm1 or H-2Kb respectively. The effect on the enhancer activity of H-2Kbm1 promoter region of the difference at position -456 has been analyzed by the chloramphenicol acetyltransferase (CAT) assay, using appropriate DdeI fragments (-533 to -408 for H-2Kbm1; -534 to -408 for H-2Kb) cloned downstream of pH-2(367)CAT gene construct. The CAT activity determined by the H-2Kbm1 fragment was about 3-fold higher than that of H-2Kb, a result which probably accounts for the higher level of the H-2Kbm1 transcript and antigen in lymph node cells. |
