| First Author | Gaston K | Year | 1994 |
| Journal | FEBS Lett | Volume | 347 |
| Issue | 2-3 | Pages | 289-94 |
| PubMed ID | 8034020 | Mgi Jnum | J:18975 |
| Mgi Id | MGI:67206 | Doi | 10.1016/0014-5793(94)00567-2 |
| Citation | Gaston K, et al. (1994) YY1 is involved in the regulation of the bi-directional promoter of the Surf-1 and Surf-2 genes. FEBS Lett 347(2-3):289-94 |
| abstractText | The Surfeit locus is an unusual cluster of at least 6 housekeeping genes whose organisation is conserved between birds and mammals. We have previously shown that the divergently transcribed Surf-1 and Surf-2 genes are separated by a bi-directional, TATA-less promoter. In mouse, the Surf-1/Surf-2 promoter contains three important factor binding sites: Su1, Su2, and Su3. These sites are conserved between the mouse and human Surf-1/Surf-2 promoters, bind nuclear factors in vitro, and are required for accurate and efficient expression of Surf-1 and Surf-2 in vivo. Using gel retardation assays, methylation interference experiments, and specific antibodies we demonstrate that the Su1 binding factor is the initiator protein YY1. Over-expression of YY1 results in a major stimulation of transcription in the Surf-1 direction and a minor stimulation of transcription in the Surf-2 direction. |
