Primary Identifier | MGI:6386244 | Allele Type | Endonuclease-mediated |
Attribute String | Epitope tag, Reporter | Gene | Cd8a |
Strain of Origin | NOD | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 genome editing is used to introduce a red fluorescent protein (tdTomato) with a 5' HA tag, an SV40 polyadenylation sequence and 3' HA tag into exon 1, following the ATG codon. The insertion of tdTomato results in a 64 bp deletion in exon 1. |