| First Author | Wang Y | Year | 2020 |
| Journal | Front Immunol | Volume | 11 |
| Pages | 581301 | PubMed ID | 33193386 |
| Mgi Jnum | J:308459 | Mgi Id | MGI:6729331 |
| Doi | 10.3389/fimmu.2020.581301 | Citation | Wang Y, et al. (2020) Natural Killer T-Cell Agonist alpha-Galactosylceramide and PD-1 Blockade Synergize to Reduce Tumor Development in a Preclinical Model of Colon Cancer. Front Immunol 11:581301 |
| abstractText | Murine and human invariant natural killer T (iNKT) lymphocytes are activated by alpha-galactosylceramide (alpha-GalCer) presented on CD1d. alpha-GalCer was first described as a lipid that had strong anti-metastatic effects in a mouse melanoma model, and it has subsequently been shown to induce efficient iNKT cell dependent tumor immunity in several tumor models. We have shown that alpha-GalCer treatment leads to a weak reduction of polyp burden in the autochthonous Apc(Min/+) mouse model for human colon cancer, however this treatment resulted in upregulation of the inhibitory receptor PD-1 on iNKT cells. While anti-PD-1 treatment can prevent immune-suppression in other cancer types, human colon cancer is generally resistant to this treatment. Here we have used the Apc(Min/+) model to investigate whether a combined treatment with alpha-GalCer and PD-1 blockade results in improved effects on polyp development. We find that PD-1 expression was high on T cells in polyps and lamina propria (LP) of Apc(Min/+) mice compared to polyp free (Apc+/+) littermates. Anti-PD-1 treatment alone promoted Tbet expression in iNKT cells and CD4 T cells, but did not significantly reduce polyp numbers. However, the combined treatment with anti-PD-1 and alpha-GalCer had synergistic effects, resulting in highly significant reduction of polyp numbers in the small and large intestine. Addition of PD-1 blockade to alpha-GalCer treatment prevented loss of iNKT cells that were skewed towards a TH1-like iNKT1 phenotype specifically in polyps. It also resulted in TH1 skewing and increased granzyme B expression of CD4 T cells. Taken together this demonstrates that a combination of immune stimulation targeting iNKT cells and checkpoint blockade may be a promising approach to develop for improved tumor immunotherapy. |
