| Primary Identifier | MGI:4835013 | Allele Type | Targeted |
| Gene | Hprt1 | Transmission | Germline |
| Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 | Is Recombinase | false |
| Is Wild Type | false | Project Collection | Pleiades Promoter Project |
| description | Germ line transmission of mutant cell line mEMS3960 has been established. |
| molecularNote | The CAG-lacZ transgene (pEMS1488) was designed with the 1627 bp CAG promoter (a human cytomegalovirus enhancer, chicken beta-actin promoter and 5' UTR, and chicken beta-actin intron/rabbit beta-globin splice acceptor, all from the pDrive-CAG vector) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. |
