| Primary Identifier | MGI:5318588 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rhd |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A vector isolated from a targeting vector library described in J:115976 was used to generate this null allele. The vector consisted of an upstream portion of the Hprt gene, a loxP site, a puromycin resistance gene, and a 7 kb genomic fragment, including exons 3 - 6, in which a 688 bp deletion was created. This vector was used in gap repair targeting where the genomic gap in the vector is repaired by the chromosomal DNA template, creating a duplication on the chromosome. Western blot analysis confirmed the absence of expression in homozygous mice. |
