Primary Identifier | MGI:5532501 | Allele Type | Targeted |
Attribute String | Inducible, Recombinase | Gene | Hprt1 |
Transmission | Germline | Strain of Origin | B6N.Cg-A<w-J> Hprt1<b-m3> |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false | Project Collection | EUCOMMTOOLS |
molecularNote | The transgene was designed with an HS4 insulator region, the 986 bp Ple265 minipromoter (based on PCP2-B; derived from a portion of the endogenous promoter (Prom 1 ONLY) from the human purkinje cell protein 2 (PCP2) locus), a chimeric intron cassette, the iCre/ERT2 fusion gene (with F3-frt sites flanking the ERT2 sequences), the mut6-WPRE element, an SV40 early polyA signal, an additional HS4 insulator region, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. |