| Primary Identifier | MGI:5906933 | Allele Type | Targeted |
| Attribute String | Inducible, Inserted expressed sequence | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | ES cell line = ZX1, which is a mutant A17 line. ZX1 ES cells are A17 ES cells engineered to carry an inducible cassette exchange allele at Hprt. A17 ES cells are mutant E14TG2A ES cells that carry an inserted rtTA sequence at the Rosa26 locus. |
| molecularNote | A p2lox targeting plasmid is designed to insert the human DUX4 ORF and 3' UTR followed by a poly A tail in front of a tetO promoter by exchanging it with the existing Cre recombinase ORF, using loxP and lox2272 sites. A non-functional neomycin resistance (neo) gene is placed downstream. This construct is inserted into a cassette exchange locus upstream of the Hprt gene using Inducible Cassette Exchange (ICE) recombination. |
