| Primary Identifier | MGI:6363976 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Gria4 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The loxP sequence and Neo cassette flanked by two frt sites were inserted into a site 331 bp upstream of exon 12. An internal ribosomal entry site (IRES) sequence and splice donor (SD) sequence from exon 8 of the mouse Hprt gene were inserted downstream of the Neo cassette for the poly-A trapping strategy. The other loxP site was introduced into a site 185 bp downstream of exon 12 to eliminate the putative transmembrane domain after Cre-mediated recombination. |
