| Primary Identifier | MGI:6459291 | Allele Type | Targeted |
| Attribute String | Conditional ready, Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The following sequences were inserted in place of the endogenous promoter, exons 1-2 and 5' end of intron 2, which are lacking in the Hprtb-m3 allele-containing ES cels: the CAG promoter, a loxP site flanked STOP cassette, sequence coding for a chimeric peptide joining the 17-amino acid F-actin-binding Lifeact peptide to monomeric EGFP, poly(A) signal sequence and human HPRT promoter and exon 1 and mouse Hprt intron 1, exon 2 and part of intron 2. Mice carrying this allele will only express the Lifact-EGFP fusion protein when crossed with mice expressing a cre recombinase allele. |
