Primary Identifier | MGI:2180846 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Cenpc1 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A construct containing a beta-geo fusion marker as well as a splice-acceptor site and a picornaviral internal ribosome-entry site (IRES) was inserted upstream of exon 8 and 9. The construct was designed to prevent the translation of the functionally essential centromere targeting region. Southern blot analysis confirmed the targeting event. |