| First Author | Kwon YK | Year | 1993 |
| Journal | Dev Biol | Volume | 158 |
| Issue | 1 | Pages | 99-100 |
| PubMed ID | 8330676 | Mgi Jnum | J:38721 |
| Mgi Id | MGI:86103 | Doi | 10.1006/dbio.1993.1170 |
| Citation | Kwon YK, et al. (1993) Proteins homologous to the Xenopus germ cell-specific RNA-binding proteins p54/p56 are temporally expressed in mouse male germ cells. Dev Biol 158(1):99-100 |
| abstractText | Antibodies specific for the Xenopus oocyte cytoplasmic 6S mRNA-binding particle p54/p56 and antibodies against Xenopus germ cell DNA-binding protein FRG Y2 recognize two RNA-binding proteins of the mouse testis. The mouse testis proteins, estimated by SDS-PAGE to be about 48 and 52 kDa, form RNA-protein complexes with either translationally regulated or control RNAs, suggesting that they are sequence-independent RNA-binding proteins. The binding of the 48/52-kDa proteins to RNA is reduced by heparin. The expression of the 48/52-kDa mouse proteins is germ cell-specific and developmentally regulated in the testis with a maximal amount of the two proteins being detected in early postmeiotic cells (round spermatids), a cell type where many mRNAs are stored. The 48/52-kDa proteins are detected solely in the nonpolysomal fractions of postmitochondrial adult testis extracts and are not detected in extracts of brain, liver, or prepuberal testes from 12-day-old mice. We conclude that two RNA-binding proteins that appear to be immunological and functional homologues of the Xenopus germ cell-specific RNA/DNA-binding proteins p54/p56/FRG Y2 are present in male germ cells and form complexes with stored mRNAs. |
