| First Author | Kitamura Y | Year | 2015 |
| Journal | Genes Cells | Volume | 20 |
| Issue | 7 | Pages | 578-89 |
| PubMed ID | 25908210 | Mgi Jnum | J:238765 |
| Mgi Id | MGI:5823556 | Doi | 10.1111/gtc.12247 |
| Citation | Kitamura Y, et al. (2015) Regulation by gut commensal bacteria of carcinoembryonic antigen-related cell adhesion molecule expression in the intestinal epithelium. Genes Cells 20(7):578-89 |
| abstractText | Carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 1 and CEACAM20, immunoglobulin superfamily members, are predominantly expressed in intestinal epithelial cells (IECs) and co-localized at the apical surface of these cells. We here showed that the expression of mouse CEACAM1 and CEACAM20 at both mRNA and protein levels was markedly reduced in IECs of the small intestine by the treatment of mice with antibiotics against Gram-positive bacteria. The expression of both proteins was also decreased in IECs of the small intestine from germ-free mice, compared with that from control specific-pathogen-free mice. Exposure of intestinal organoids to IFN-gamma markedly increased the expression of either CEACAM1 or CEACAM20, whereas the exposure to TNF-alpha increased the expression of the former protein, but not that of the latter. In contrast, the expression of CEACAM20, but not of CEACAM1, in intestinal organoids was markedly increased by exposure to butyrate, a short-chain fatty acid produced by bacterial fermentation in the intestine. Collectively, our results suggest that Gram-positive bacteria promote the mRNA expression of CEACAM1 or CEACAM20 in the small intestine. Inflammatory cytokines or butyrate likely participates in such effects of commensal bacteria. |
