Primary Identifier | MGI:6850615 | Allele Type | Endonuclease-mediated |
Attribute String | Conditional ready | Gene | Nras |
Strain of Origin | B6.Cg-Tg(Tyr-cre/ERT2)13Bos Nras<em5Burd>/Mmnc | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR-targeting produced the amino acid substitution of glycine to aspartic acid at position 13 (G12D). A floxed stop cassette in intron 1 prevents expression unless removed by cre-mediated recombination. A silent G to A mutation occurred in the third nucleotide of codon 15. |