| First Author | Matteson KJ | Year | 1986 |
| Journal | J Clin Endocrinol Metab | Volume | 63 |
| Issue | 3 | Pages | 789-91 |
| PubMed ID | 3488328 | Mgi Jnum | J:8362 |
| Mgi Id | MGI:56829 | Doi | 10.1210/jcem-63-3-789 |
| Citation | Matteson KJ, et al. (1986) Assignment of the gene for adrenal P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase) to human chromosome 10. J Clin Endocrinol Metab 63(3):789-91 |
| abstractText | P450c17 is the single enzyme mediating both 17 alpha-hydroxylase and 17,20 lyase activities. We identified several human P450c17 cDNA clones in a human adrenal cDNA library we constructed in lambda gt10. A short clone containing the 3'-terminal 650 bases of the full-length sequence was used to examine Southern blots of DNA from normal persons and from a panel of mouse/human somatic cell hybrid lines. The pattern of hybridization of this cDNA to normal human DNA cut with 8 restriction endonucleases suggests the human genome has two (or more) P450c17 genes. The pattern of hybridization to the somatic cell hybrid cell lines, each containing a limited, known number of human chromosomes, indicates the human adrenal P450c17 gene lies on chromosome 10. The chromosomal locations of the other P450c17 genes could not be determined. |
