| First Author | Yu SM | Year | 2019 |
| Journal | J Biol Chem | Volume | 294 |
| Issue | 7 | Pages | 2500-2518 |
| PubMed ID | 30538132 | Mgi Jnum | J:277075 |
| Mgi Id | MGI:6304853 | Doi | 10.1074/jbc.RA118.004926 |
| Citation | Yu SM, et al. (2019) The deubiquitinase ubiquitin-specific protease 20 is a positive modulator of myocardial beta1-adrenergic receptor expression and signaling. J Biol Chem 294(7):2500-2518 |
| abstractText | Reversible ubiquitination of G protein-coupled receptors regulates their trafficking and signaling; whether deubiquitinases regulate myocardial beta1-adrenergic receptors (beta1ARs) is unknown. We report that ubiquitin-specific protease 20 (USP20) deubiquitinates and attenuates lysosomal trafficking of the beta1AR. beta1AR-induced phosphorylation of USP20 Ser-333 by protein kinase A-alpha (PKAalpha) was required for optimal USP20-mediated regulation of beta1AR lysosomal trafficking. Both phosphomimetic (S333D) and phosphorylation-impaired (S333A) USP20 possess intrinsic deubiquitinase activity equivalent to WT activity. However, unlike USP20 WT and S333D, the S333A mutant associated poorly with the beta1AR and failed to deubiquitinate the beta1AR. USP20-KO mice showed normal baseline systolic function but impaired beta1AR-induced contractility and relaxation. Dobutamine stimulation did not increase cAMP in USP20-KO left ventricles (LVs), whereas NKH477-induced adenylyl cyclase activity was equivalent to WT. The USP20 homolog USP33, which shares redundant roles with USP20, had no effect on beta1AR ubiquitination, but USP33 was up-regulated in USP20-KO hearts suggesting compensatory regulation. Myocardial beta1AR expression in USP20-KO was drastically reduced, whereas beta2AR expression was maintained as determined by radioligand binding in LV sarcolemmal membranes. Phospho-USP20 was significantly increased in LVs of wildtype (WT) mice after a 1-week catecholamine infusion and a 2-week chronic pressure overload induced by transverse aortic constriction (TAC). Phospho-USP20 was undetectable in beta1AR KO mice subjected to TAC, suggesting a role for USP20 phosphorylation in cardiac response to pressure overload. We conclude that USP20 regulates beta1AR signaling in vitro and in vivo Additionally, beta1AR-induced USP20 phosphorylation may serve as a feed-forward mechanism to stabilize beta1AR expression and signaling during pathological insults to the myocardium. |
