| First Author | Bertling E | Year | 2021 |
| Journal | EMBO Rep | Volume | 22 |
| Issue | 4 | Pages | e50145 |
| PubMed ID | 33719157 | Mgi Jnum | J:311404 |
| Mgi Id | MGI:6711942 | Doi | 10.15252/embr.202050145 |
| Citation | Bertling E, et al. (2021) Carbonic anhydrase seven bundles filamentous actin and regulates dendritic spine morphology and density. EMBO Rep 22(4):e50145 |
| abstractText | Intracellular pH is a potent modulator of neuronal functions. By catalyzing (de)hydration of CO2 , intracellular carbonic anhydrase (CAi ) isoforms CA2 and CA7 contribute to neuronal pH buffering and dynamics. The presence of two highly active isoforms in neurons suggests that they may serve isozyme-specific functions unrelated to CO2 -(de)hydration. Here, we show that CA7, unlike CA2, binds to filamentous actin, and its overexpression induces formation of thick actin bundles and membrane protrusions in fibroblasts. In CA7-overexpressing neurons, CA7 is enriched in dendritic spines, which leads to aberrant spine morphology. We identified amino acids unique to CA7 that are required for direct actin interactions, promoting actin filament bundling and spine targeting. Disruption of CA7 expression in neocortical neurons leads to higher spine density due to increased proportion of small spines. Thus, our work demonstrates highly distinct subcellular expression patterns of CA7 and CA2, and a novel, structural role of CA7. |
