First Author | Ihle JN | Year | 1982 |
Journal | J Immunol | Volume | 129 |
Issue | 6 | Pages | 2431-6 |
PubMed ID | 6815268 | Mgi Jnum | J:14592 |
Mgi Id | MGI:62756 | Doi | 10.4049/jimmunol.129.6.2431 |
Citation | Ihle JN, et al. (1982) Procedures for the purification of interleukin 3 to homogeneity. J Immunol 129(6):2431-6 |
abstractText | A procedure is described for the routine purification of IL 3 to homogeneity from WEHI-3-conditioned media. The techniques employed include ammonium sulfate fractionation, DEAE-cellulose, hydroxylapatite, and G-75 Sephadex column chromatography. The last step in purification involves chromatography on C18 hydrophobic supports in RP-HPLC systems, which results in the coelution of a protein peak and IL 3 activity. This purification sequence results in approximately a 1,000,000-fold purification from the initial starting material with yields of 5 to 10% of the initial activity. typically, 150 liters of conditioned media yields 2 to 10 micrograms of IL 3. The purified material was homogeneous by SDS-PAGE analysis and had an apparent m.w. of 28,000. Purified IL 3 had a specific activity of approximately 0.05 ng/unit of activity. Additional criteria used to establish the relationship of the 28,000-dalton protein to IL 3 include the ability of an antiserum against IL 3 to concomitantly immunoprecipitate the iodinated protein and to inhibit its biologic activity as well as the ability of the iodinated protein to bind specifically to cell lines known to require IL 3 for growth. |