| Primary Identifier | MGI:5811610 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Camk2a |
| Strain of Origin | C57BL/6J x DBA/2J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using the CRISPR/Cas9 system, the Glu183 codon GAA was targeted and changed to valine codon GTG with the aid of a donor DNA sequence and sgRNA CTCCCCAGAAGTGCTGAGGAAAG. This mutation, in the catalytic domain of the gene, models a novel mutation in a human autism spectrum disorder (ASD) patient. Immunoblots showed a marked reduction in peptide levels for this gene in the forebrain of hetero- and homozygous mutants. |
