First Author | Gonda TJ | Year | 1985 |
Journal | EMBO J | Volume | 4 |
Issue | 8 | Pages | 2003-8 |
PubMed ID | 2998780 | Mgi Jnum | J:17641 |
Mgi Id | MGI:65674 | Doi | 10.1002/j.1460-2075.1985.tb03884.x |
Citation | Gonda TJ, et al. (1985) Nucleotide sequence of cDNA clones of the murine myb proto-oncogene. EMBO J 4(8):2003-8 |
abstractText | We have isolated cDNA clones of murine c-myb mRNA which contain approximately 2.8 kb of the 3.9-kb mRNA sequence. Nucleotide sequencing has shown that these clones extend both 5' and 3' to sequences homologous to the v-myb oncogenes of avian myeloblastosis virus and avian leukemia virus E26. The sequence contains an open reading frame of 1944 nucleotides, and could encode a protein which is both highly homologous, and of similar size (71 kd), to the chicken c-myb protein. Examination of the deduced amino acid sequence of the murine c-myb protein revealed the presence of a 3-fold tandem repeat of 52 residues near the N terminus of the protein, and has enabled prediction of some of the likely structural features of the protein. These include a high alpha-helix content, a basic region toward the N terminus of the protein and an overall globular configuration. The arrangement of genomic c-myb sequences, detected using the cDNA clones as probes, was compared with the reported structure of rearranged c-myb in certain tumour cells. This comparison suggested that the rearranged c-myb gene may encode a protein which, like the v-myb protein, lacks the N-terminal region of c-myb. |