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Publication : Rat ligandin mRNA molecular cloning and sequencing.

First Author  Daniel V Year  1983
Journal  Arch Biochem Biophys Volume  227
Issue  1 Pages  266-71
PubMed ID  6688942 Mgi Jnum  J:14775
Mgi Id  MGI:62937 Doi  10.1016/0003-9861(83)90370-3
Citation  Daniel V, et al. (1983) Rat ligandin mRNA molecular cloning and sequencing. Arch Biochem Biophys 227(1):266-71
abstractText  Recombinant plasmids containing the double-stranded cDNA sequences of mRNA for the Mr 22,000 ligandin (glutathione S-transferase B) subunit (Ya) have been constructed. The DNA sequence of an insert corresponding to the middle and 3' regions of the mRNA was determined and an amino acid sequence was proposed for the ligandin Ya subunit. The proposed sequence reveals a high content of basic amino acids (Arg and Lys) and Leu, is consistent with the amino acid composition, and predicts the correct number of peptides derived from tryptic digests reported for ligandin.
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