| First Author | Subramanian BC | Year | 2020 |
| Journal | J Cell Biol | Volume | 219 |
| Issue | 10 | PubMed ID | 32854115 |
| Mgi Jnum | J:306353 | Mgi Id | MGI:6706152 |
| Doi | 10.1083/jcb.201910215 | Citation | Subramanian BC, et al. (2020) The LTB4-BLT1 axis regulates actomyosin and beta2-integrin dynamics during neutrophil extravasation. J Cell Biol 219(10) |
| abstractText | The eicosanoid leukotriene B4 (LTB4) relays chemotactic signals to direct neutrophil migration to inflamed sites through its receptor BLT1. However, the mechanisms by which the LTB4-BLT1 axis relays chemotactic signals during intravascular neutrophil response to inflammation remain unclear. Here, we report that LTB4 produced by neutrophils acts as an autocrine/paracrine signal to direct the vascular recruitment, arrest, and extravasation of neutrophils in a sterile inflammation model in the mouse footpad. Using intravital subcellular microscopy, we reveal that LTB4 elicits sustained cell polarization and adhesion responses during neutrophil arrest in vivo. Specifically, LTB4 signaling coordinates the dynamic redistribution of non-muscle myosin IIA and beta2-integrin, which facilitate neutrophil arrest and extravasation. Notably, we also found that neutrophils shed extracellular vesicles in the vascular lumen and that inhibition of extracellular vesicle release blocks LTB4-mediated autocrine/paracrine signaling required for neutrophil arrest and extravasation. Overall, we uncover a novel complementary mechanism by which LTB4 relays extravasation signals in neutrophils during early inflammation response. |
