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Publication : Identification and characterization of cDNAs encoding four novel proteins that interact with translin associated factor-X.

First Author  Bray JD Year  2002
Journal  Genomics Volume  79
Issue  6 Pages  799-808
PubMed ID  12036294 Mgi Jnum  J:76035
Mgi Id  MGI:2181479 Doi  10.1006/geno.2002.6779
Citation  Bray JD, et al. (2002) Identification and Characterization of cDNAs Encoding Four Novel Proteins that Interact with Translin Associated Factor-X. Genomics 79(6):799-808
abstractText  Translin-associated factor X (TRAX) is the predominantly cytoplasmic binding partner of TB-RBP/ translin in mouse testis. Four mouse testis cDNAs encoding specific TRAX-interacting proteins were isolated from a yeast two-hybrid library screen. One novel cDNA designated Tsnaxip1 (TRAX- interacting protein-1) encodes 709 amino acids. We isolated a cDNA encoding the 427 carboxy-terminal amino acids of MEA-2, a Golgi-associated, maleenhanced autoantigen; a cDNA encoding 429 amino acids with 73% homology to centrosomal Akap9; and a cDNA encoding 346 amino acids with 75% homology to SUN1, a predicted human protein that contains a SUN domain (which is present in some perinuclear proteins). Interactions were verified using in vitro synthesized fusion proteins. All four genes were expressed in the testis and enriched in germ cells. Confocal microscopy studies using green fluorescent protein fusion proteins determined that these TRAX-interacting proteins colocalize with TRAX. The data suggest that TRAX may have a function associated with perinuclear organelles during spermatogenesis. (c)2002 Elsevier Science (USA).
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