| First Author | Brady KP | Year | 1997 |
| Journal | Genome Res | Volume | 7 |
| Issue | 11 | Pages | 1085-93 |
| PubMed ID | 9371744 | Mgi Jnum | J:44483 |
| Mgi Id | MGI:1100270 | Doi | 10.1101/gr.7.11.1085 |
| Citation | Brady KP, et al. (1997) Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism analysis. Genome Res 7(11):1085-93 |
| abstractText | We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3'-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism. |
