| First Author | Choong ML | Year | 2003 |
| Journal | FEBS Lett | Volume | 550 |
| Issue | 1-3 | Pages | 155-62 |
| PubMed ID | 12935903 | Mgi Jnum | J:129497 |
| Mgi Id | MGI:3769458 | Doi | 10.1016/s0014-5793(03)00844-5 |
| Citation | Choong ML, et al. (2003) A novel role for proliferin-2 in the ex vivo expansion of hematopoietic stem cells. FEBS Lett 550(1-3):155-62 |
| abstractText | A family of proliferin genes was discovered on a microarray analysis of hematopoiesis supportive stromal cell lines. Proliferin-2 (PLF2) increased the frequency of long-term culture-initiating cells (LTC-IC) from 1 in 340 to 1 in 256 of the primary hematopoietic stem cell (HSC)-enriched bone marrow cells grown on MS5.1 feeder layer. A repeat using AFT024 feeder layer also showed a similar increase in LTC-IC (from 1 in 386 cells to 1 in 260 cells). The clonogenic output of the LTC-ICs was also increased significantly. The growth of various hematopoietic and stromal cell lines treated with PLF2 was found to increase by 4-27%, as measured by cell count and DNA synthesis assay. These findings open up the possibility of using PLF2 as a new member of the growth factor cocktails for the ex vivo expansion of HSC. |
