| First Author | Chen YC | Year | 2023 |
| Journal | J Am Soc Nephrol | Volume | 34 |
| Issue | 7 | Pages | 1159-1165 |
| PubMed ID | 37094382 | Mgi Jnum | J:359643 |
| Mgi Id | MGI:7788517 | Doi | 10.1681/ASN.0000000000000148 |
| Citation | Chen YC, et al. (2023) Molecular MR Imaging of Renal Fibrogenesis in Mice. J Am Soc Nephrol 34(7):1159-1165 |
| abstractText | BACKGROUND: In most CKDs, lysyl oxidase oxidation of collagen forms allysine side chains, which then form stable crosslinks. We hypothesized that MRI with the allysine-targeted probe Gd-oxyamine (OA) could be used to measure this process and noninvasively detect renal fibrosis. METHODS: Two mouse models were used: hereditary nephritis in Col4a3-deficient mice (Alport model) and a glomerulonephritis model, nephrotoxic nephritis (NTN). MRI measured the difference in kidney relaxation rate, DeltaR1, after intravenous Gd-OA administration. Renal tissue was collected for biochemical and histological analysis. RESULTS: DeltaR1 was increased in the renal cortex of NTN mice and in both the cortex and the medulla of Alport mice. Ex vivo tissue analyses showed increased collagen and Gd-OA levels in fibrotic renal tissues and a high correlation between tissue collagen and DeltaR1. CONCLUSIONS: Magnetic resonance imaging using Gd-OA is potentially a valuable tool for detecting and staging renal fibrogenesis. |
